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KMID : 0043319940170050354
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Archives of Pharmacal Research
1994 Volume.17 No. 5 p.354 ~ p.359
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Inhibition of C-terminal O-Methyltransferase by a Rat Liver Cytosolic Peptide
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Park Seung-Hee
Lee Hyang-Woo
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Abstract
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The activity of SD-framesylcysteine O-methyltransferase was assayed by incubating the enzyrne with a synthetic in vitro substrate, [N-acetyl-S-trans, trns-famesyl-L-cysteine (AFC)], together with S-adenosyl-L-[emthyl-C)ester(AFCME)], was then analyzed either directly on HPLC or by converting the AFC[]ME to [] aclcohol by basehydrolysis. Employing these two analytical methods, it was established that a peptide purifed from rat liver cytosol fraction [Int. J. Biochem., 25, 1157 919930] strongly inhibited the above enzyme activity with M. Also, the S-famesylcysteine O-methyltransferase from several human colon cancer cells was equally inhibited by the peptide.
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KEYWORD
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Peptide inhibitor, S-famesylcysteine O-methyltransferase
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